• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    A diagnostic agent for the detection of occult blood in feces comprising hydrogen peroxide and, as a chromogen, guaiaconic acid A, optionally with a stabilizer. The invention provides, as a novel substance, guaiaconic acid A having a specific extinction E1 cm1% at 600 nm of at least 200, determined by the reaction with peroxidase and hydrogen peroxide, having infra-red bands at 1600 cm-1
    公开号:SU854274A3
    申请号:SU782600100
    申请日:1978-04-07
    公开日:1981-08-07
    发明作者:Гютляйн Вернер；Вилингер Ханс；Риттерсдорф Вальтер；Вернер ВОЛЬФГАНГ
    申请人:Берингер Маннхайм Гмбх (Фир-Ma)；
    IPC主号:
    专利说明:

    (54) METHOD FOR OBTAINING FRY GUYAK RESIN
    The invention relates to the field of organic chemistry, in particular to a method for producing a fraction of guaclylated resin used in medicine in the case of diseases of the digestive organs, resulting in occult blood in the stool, the detection of which is an important point in establishing the correct diagnosis. . The famous gwa cove is a resin that is extracted from the core of the tropical tree Guajacum officinal is. sanktum is used to determine hidden blood in the feces. However, frequent use of gum resin results in erroneous results. The analysis is carried out by treating the suspension with hydrogen peroxide and an alcoholic solution of gum resin. The occurrence of naked coloration indicates the presence of occult blood. Two major components of the resin, namely, furanumine, and also guva conic acid 1 are responsible for the appearance of the colouration. However, guava resin does not always contain the components contained in it in the same ratio, often unstable components are formed that can decompose and thus reduce the accuracy and reproducibility of the analysis. At the same time, the isolation and application of the two main components in an individual form is difficult, since the use of the furgucine does not give stable results, and it is possible to carry out the isolation of guanic acid only by thin-layer chromatography with a very low yield. The purpose of the invention is to develop a production method A new, stable mixture of components of gaqua gum — fractions of gaqua gum, suitable for diagnosing the presence of blood in the feces. The delivered gel is obtained by the method of obtaining the fraction of guaclylated resin, which consists in the fact that guvac resin isolated from the core of a tropical tree Guajacum offtcinalls and Guajacum sanktum is dissolved in acetone, then toluene is added, then the mixture is filtered, the filtrate is evaporated to dryness and the residue dissolved in a mixture of n-heptane and ethyl acetate in a ratio of 2: 5 or in acetone and
    权利要求:
    Claims (1)
    [1]
    Claim
    A method for producing a fraction of guaiac gum, characterized in that the guaiac gum isolated 4Q from the core of the tropical tree Guajacum officinalis and Guajacum sanktum is dissolved in acetone, then toluene is added, after which the resulting mixture is filtered, the filtrate is evaporated to dryness and the residue is dissolved in n-heptane mixtures and acetic ether in a ratio of 2: 5 or in acetone and separated on a silica gel column in a carbon dioxide atmosphere using an eluent, which is used as a mixture of n-heptane and acetic ether in a ratio of 2: 5 or a mixture of acetone and tolu la in a ratio of 1: 5, whereby the fractions with Rf 0,45 in toluene, dioxane, glacial acetic acid in a ratio of 90:25:10, was evaporated to dryness and their resulting residue was crystallized from p-xylene.
    类似技术:
    公开号 | 公开日 | 专利标题
    SU854274A3|1981-08-07|Method of producing guaiac
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    同族专利:
    公开号 | 公开日
    CH636625A5|1983-06-15|
    FR2386556A1|1978-11-03|
    AT358743B|1980-09-25|
    DE2716061C2|1982-03-25|
    DE2716061A1|1978-10-19|
    IT1095395B|1985-08-10|
    ATA248678A|1980-02-15|
    CA1115724A|1982-01-05|
    US4219336A|1980-08-26|
    JPS588400B2|1983-02-15|
    IT7822063D0|1978-04-06|
    JPS53127812A|1978-11-08|
    SE7803890L|1978-10-10|
    AR216788A1|1980-01-31|
    US4297271A|1981-10-27|
    FI781014A|1978-10-10|
    BE865700A|1978-10-05|
    NL7803721A|1978-10-11|
    FR2386556B1|1982-11-05|
    HU180211B|1983-02-28|
    DD135768A5|1979-05-23|
    BR7802179A|1978-12-19|
    GB1571362A|1980-07-16|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    US2258673A|1940-02-07|1941-10-14|Nat Oil Prod Co|Process for treating fat-soluble vitamin-containing materials|
    US2345098A|1941-07-26|1944-03-28|Nat Oil Prod Co|Process of saponifying vitamin-containing fatty material|
    US2529446A|1948-07-02|1950-11-07|Swift & Co|Gum guaiac treatment|
    DK153334C|1974-01-09|1988-11-14|Shionogi & Co|DIAGNOSTIC PREPARATIONS|
    JPS5263794A|1975-11-21|1977-05-26|Shionogi Seiyaku Kk|Test piece for latent blood|
    FI52256C|1975-12-08|1977-07-11|Osmo Antero Suovaniemi|Sampling device for transport and analysis of samples.|JPS6236680B2|1980-02-21|1987-08-07|Toyo Jozo Kk|
    US4526869A|1980-09-24|1985-07-02|Regents Of The University Of Minnesota|Method for quantitatively determining the concentration of hemoglobin in a biological sample|
    ZA829434B|1982-01-05|1984-05-30|Hematec Corp|Home diagnostic aid and method for determining the presence of occult blood for use by the general public|
    US4956300A|1982-01-05|1990-09-11|Helena Laboratories Corporation|Aid for determining the presence of occult blood, method of making the aid, and method of using the aid|
    ZA831268B|1982-04-30|1984-10-31|Hematec Corp|Home diagnostic aid and method for determining the presence of occult blood for use by the general public|
    PH23572A|1982-05-28|1989-09-11|Smithkline Diagnostics Inc|Specimen test slide for occult blood testing|
    US4725553A|1983-09-29|1988-02-16|Helena Laboratories Corporation|Test composition for detecting occult blood|
    US4541987A|1983-09-29|1985-09-17|Helena Laboratories Corporation|Test pad for detecting occult blood|
    US5702913A|1983-12-21|1997-12-30|Helena Laboratories Corporation|Chromgen-reagent test system|
    US5273888A|1984-01-16|1993-12-28|Helena Laboratories Corporation|Chemical test kit and method for determining the presence of blood in a specimen and for verifying the effectiveness of the chemicals|
    US4676950A|1984-02-03|1987-06-30|Foster Research Corporation|Indicator and test device for detecting occult blood|
    US4615982A|1984-12-11|1986-10-07|Lawrence Paul J|Fecal occult blood test|
    US4818702A|1986-06-02|1989-04-04|Litmus Concepts, Inc.|Fecal occult blood test reagent|
    US5219554A|1986-07-03|1993-06-15|Advanced Magnetics, Inc.|Hydrated biodegradable superparamagnetic metal oxides|
    JPS63223096A|1987-03-11|1988-09-16|Dainippon Printing Co Ltd|Purified quaiac oil and its production|
    JP2589079B2|1987-03-11|1997-03-12|大日本印刷株式会社|Method for producing purified guaiac fat|
    US5081040A|1987-06-29|1992-01-14|Helena Laboratories Corporation|Composition and kit for testing for occult blood in human and animal excretions, fluids, or tissue matrixes|
    JP2660558B2|1988-08-17|1997-10-08|大日本印刷株式会社|Purified guaiac fat and process for producing the same|
    US5182191A|1988-10-14|1993-01-26|Pacific Biotech, Inc.|Occult blood sampling device and assay|
    US5196167A|1989-04-04|1993-03-23|Helena Laboratories Corporation|Fecal occult blood test product with positive and negative controls|
    US5217874A|1989-04-04|1993-06-08|Helena Laboratories Corporation|Fecal occult blood test product with positive and negative controls|
    WO1994015594A1|1993-01-11|1994-07-21|Tsumura & Co.|Vascularization inhibitor and novel compound|
    US5447868A|1993-09-14|1995-09-05|Propper Manufacturing Co. Inc.|Method, reagent and kit for the detection of fecal occult blood|
    US6309887B1|1998-01-27|2001-10-30|Flexsite Diagnostics, Inc.|Filter paper treatment for improved diagnostic assays|
    US7109038B2|2002-06-13|2006-09-19|The Johns Hopkins University|Occult blood detection in biological samples by laser desorption and matrix-assisted laser desorption/ionization mass spectrometry for biomedical applications|
    US7195878B2|2004-09-30|2007-03-27|Cryo-Genomics|Device and method for fecal testing|
    US9091682B1|2014-05-01|2015-07-28|Steven M Hacker|Tissue specimen bottle with color indicator in lid verifying and confirming presence of human tissue or blood contained in specimen bottle|
    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    DE2716061A|DE2716061C2|1977-04-09|1977-04-09|Guaiaconic acid A, process for its preparation and the use of this substance as a diagnostic agent|
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